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dermal cell basal medium plus keratinocyte growth kit  (ATCC)


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    Structured Review

    ATCC dermal cell basal medium plus keratinocyte growth kit
    Dermal Cell Basal Medium Plus Keratinocyte Growth Kit, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 437 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dermal+cell+basal+medium+plus+keratinocyte+growth+kit/Dermal+Cell+Basal+Medium/pm42276073-407-9-17
    Average 96 stars, based on 437 article reviews
    dermal cell basal medium plus keratinocyte growth kit - by Bioz Stars, 2026-10
    96/100 stars

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    other:

    Article Title: Delivery and formulation of engineered nucleic acids
    Article Snippet: A data acquisition system (Intellution Fix 32, OSIsoft, LLC, San Leandro, CA) recorded temperature, pH, and dissolved oxygen (DO) throughout runs.

    Article Title: Mammalian somatic cell with modified RNA encoding reprogramming factors
    Article Snippet: Completed reactions were purified on QIAquick columns (Qiagen), and analyzed in 20 uL qPCRs, each templated with ̃10% of the total cDNA prep.

    Transfection:

    Article Title: Delivery and formulation of engineered nucleic acids
    Article Snippet: .. RNA transfections are performed in Nutristem xenofree hES media (STEMGENT®, Cambridge, MA) for RiPS derivations, Dermal Cell Basal Medium plus Keratinocyte Growth Kit (ATCC) for keratinocyte experiments, and Opti-MEM plus 2% FBS for all other experiments. ..

    Article Title: Delivery and formulation of engineered nucleic acids
    Article Snippet: .. RNA transfections are performed in Nutristem xenofree hES media (STEMGENT®, Cambridge, MA) for RiPS derivations, Dermal Cell Basal Medium plus Keratinocyte Growth Kit (ATCC) for keratinocyte experiments, and Opti-MEM plus 2% FBS for all other experiments. ..

    Article Title: Delivery and formulation of engineered nucleic acids
    Article Snippet: .. RNA transfections are performed in Nutristem xenofree hES media (STEMGENT®, Cambridge, MA) for RiPS derivations, Dermal Cell Basal Medium plus Keratinocyte Growth Kit (ATCC) for keratinocyte experiments, and Opti-MEM plus 2% FBS for all other experiments. ..

    Article Title: Delivery and formulation of engineered nucleic acids
    Article Snippet: .. RNA transfections are performed in Nutristem xenofree hES media (STEMGENT®, Cambridge, MA) for RiPS derivations, Dermal Cell Basal Medium plus Keratinocyte Growth Kit (ATCC) for keratinocyte experiments, and Opti-MEM plus 2% FBS for all other experiments. ..

    Cell Culture:

    Article Title: SenCat: Cataloging human cell senescence through multiomic profiling of multiple senescent primary cell types
    Article Snippet: HSAEC lung epithelial cells (ATCC, PCS-301-010) were cultured using an Airway Epithelial Cell Basal Medium plus Bronchial Epithelial Cell Growth Kit (ATCC). .. HEKn epidermal skin keratinocytes (ATCC, PCS-200-010) were cultured in Dermal Cell Basal Medium plus Keratinocyte Growth Kit (ATCC). .. HCAEC coronary artery endothelial cells (LifeLine Cell Technology, FC-0032) were cultured in VascuLife EnGS Endothelial Medium Complete Kit (LifeLine).

    Article Title: SenCat: Cataloging human cell senescence through multi-omic profiling of multiple senescent primary cell types.
    Article Snippet: HSAEC lung epithelial cells (ATCC, PCS-301010) were cultured using an Airway Epithelial Cell Basal Medium plus Bronchial Epithelial Cell Growth Kit (ATCC). .. HEKn epidermal skin keratinocytes (ATCC, PCS-200-010) were cultured in Dermal Cell Basal Medium plus Keratinocyte Growth Kit (ATCC). .. HCAEC coronary artery endothelial cells (LifeLine Cell Technology, FC-0032) were cultured in VascuLife EnGS Endothelial Medium Complete Kit (LifeLine).



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    96
    ATCC dermal cell basal medium plus keratinocyte growth kit
    Dermal Cell Basal Medium Plus Keratinocyte Growth Kit, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dermal+cell+basal+medium+plus+keratinocyte+growth+kit/Dermal+Cell+Basal+Medium/pm42276073-407-9-17
    Average 96 stars, based on 1 article reviews
    dermal cell basal medium plus keratinocyte growth kit - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    95
    ATCC dermal cell basal medium plus keratinocyte growth supplement kit
    FIGURE 1 Elevated glucose concentration induces viability and migration of highly glycolytic oral dysplastic <t>keratinocytes.</t> (A) Metabolic potential of indicated cells tested with the Agilent Seahorse XFp Cell Energy Phenotype assay. Open square baseline; closed square-stress condition. OCR: Oxygen consumption rate; ECAR: Extracellular acidification. (B) Basal glycolysis analyzed with Seahorse glycolytic rate assay in indicated cells (glycoPER: Glycolytic proton efflux rate). Data represent mean ± S.E.M., *p < 0.001. (C) Whole-cell extracts from PGK, LEUK1, and DOK were subjected to western blotting for GLUT1 and HKII. β-Actin served as loading control. (D) DOK were exposed to normal (5 mM) or high glucose (20 mM)-containing media for 72 h. Then, MTS reagent was added to evaluate cell viability. Data represent mean ± S.E.M. *p < 0.0001 versus 5 mM glucose. (E) Representative image showing DOK migration following exposure to normal or high glucose. Acellular gap closure was quantified relative to time 0 as depicted by the dotted white line (F). Data represent mean ± S.E.M. *p < 0.01 versus 5 mM glucose.
    Dermal Cell Basal Medium Plus Keratinocyte Growth Supplement Kit, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dermal+cell+basal+medium+plus+keratinocyte+growth+kit/Dermal+Cell+Basal+Medium/pm37817274-40-12-21
    Average 95 stars, based on 1 article reviews
    dermal cell basal medium plus keratinocyte growth supplement kit - by Bioz Stars, 2026-10
    95/100 stars
      Buy from Supplier

    Image Search Results


    FIGURE 1 Elevated glucose concentration induces viability and migration of highly glycolytic oral dysplastic keratinocytes. (A) Metabolic potential of indicated cells tested with the Agilent Seahorse XFp Cell Energy Phenotype assay. Open square baseline; closed square-stress condition. OCR: Oxygen consumption rate; ECAR: Extracellular acidification. (B) Basal glycolysis analyzed with Seahorse glycolytic rate assay in indicated cells (glycoPER: Glycolytic proton efflux rate). Data represent mean ± S.E.M., *p < 0.001. (C) Whole-cell extracts from PGK, LEUK1, and DOK were subjected to western blotting for GLUT1 and HKII. β-Actin served as loading control. (D) DOK were exposed to normal (5 mM) or high glucose (20 mM)-containing media for 72 h. Then, MTS reagent was added to evaluate cell viability. Data represent mean ± S.E.M. *p < 0.0001 versus 5 mM glucose. (E) Representative image showing DOK migration following exposure to normal or high glucose. Acellular gap closure was quantified relative to time 0 as depicted by the dotted white line (F). Data represent mean ± S.E.M. *p < 0.01 versus 5 mM glucose.

    Journal: Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology

    Article Title: Glucose upregulates amphiregulin in oral dysplastic keratinocytes: A potential role in diabetes-associated oral carcinogenesis.

    doi: 10.1111/jop.13493

    Figure Lengend Snippet: FIGURE 1 Elevated glucose concentration induces viability and migration of highly glycolytic oral dysplastic keratinocytes. (A) Metabolic potential of indicated cells tested with the Agilent Seahorse XFp Cell Energy Phenotype assay. Open square baseline; closed square-stress condition. OCR: Oxygen consumption rate; ECAR: Extracellular acidification. (B) Basal glycolysis analyzed with Seahorse glycolytic rate assay in indicated cells (glycoPER: Glycolytic proton efflux rate). Data represent mean ± S.E.M., *p < 0.001. (C) Whole-cell extracts from PGK, LEUK1, and DOK were subjected to western blotting for GLUT1 and HKII. β-Actin served as loading control. (D) DOK were exposed to normal (5 mM) or high glucose (20 mM)-containing media for 72 h. Then, MTS reagent was added to evaluate cell viability. Data represent mean ± S.E.M. *p < 0.0001 versus 5 mM glucose. (E) Representative image showing DOK migration following exposure to normal or high glucose. Acellular gap closure was quantified relative to time 0 as depicted by the dotted white line (F). Data represent mean ± S.E.M. *p < 0.01 versus 5 mM glucose.

    Article Snippet: Primary human-derived gingival keratinocytes (PGK, ATCC) were grown and maintained in a dermal cell basal medium plus keratinocyte growth supplement kit (ATCC).

    Techniques: Concentration Assay, Migration, Western Blot, Control

    FIGURE 6 Proposed model for the effects of high glucose-induced AREG/EGFR activation on promoting aerobic glycolysis and increasing cell migration in oral dysplastic keratinocytes. High glucose uptake by GLUT1 transporters upregulates AREG expression and secretion leading to EGFR activation. AREG/EGFR signaling sustains a glycolytic phenotype as evidenced by increased PKM2 expression and lactate production to enhance cell migration in yet to be elucidated mechanisms.

    Journal: Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology

    Article Title: Glucose upregulates amphiregulin in oral dysplastic keratinocytes: A potential role in diabetes-associated oral carcinogenesis.

    doi: 10.1111/jop.13493

    Figure Lengend Snippet: FIGURE 6 Proposed model for the effects of high glucose-induced AREG/EGFR activation on promoting aerobic glycolysis and increasing cell migration in oral dysplastic keratinocytes. High glucose uptake by GLUT1 transporters upregulates AREG expression and secretion leading to EGFR activation. AREG/EGFR signaling sustains a glycolytic phenotype as evidenced by increased PKM2 expression and lactate production to enhance cell migration in yet to be elucidated mechanisms.

    Article Snippet: Primary human-derived gingival keratinocytes (PGK, ATCC) were grown and maintained in a dermal cell basal medium plus keratinocyte growth supplement kit (ATCC).

    Techniques: Activation Assay, Migration, Expressing